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. 2018 Aug 24;9:3424. doi: 10.1038/s41467-018-05952-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — 3D structural features and volume fractions. All panels labeled with (i) represent M. galloprovincialis, (ii) M. californianus, (iii) S. bifurcatus, and (iv) M. capax. a Reconstructed tomographic representations of the secretory vesicles and encapsulated structures (scale bar = 500 nm); b fitted size distributions of M. galloprovincialis (red, n = 122), M. californianus (blue, n = 176), and S. bifurcatus (green, n = 102) as deduced from TEM and electron tomographic investigations. c Granular volume fractions from the secretory vesicles (blue) compared to those of the cuticle (green, see Supplementary Figure 7 for full datasets), as well as normalized to the total thread size (d), show an increase in granular density after thread formation. Note that the intergranular matrix space in M. galloprovincialis is taken into account as well (dazzled segments, n = four tomographic sections, technical replicates, 1 µm × 1 µm × 60 Z slices, mean ± s.e.m.)