Fig. 5.
M-STED of replication foci in MCF10A nuclei. a, e Examples of acquired stack images, from the confocal F1 to maximum STED power F8 (Pmax = 19 mW). Depicted is the sum of the last four frames F5–8. Color scale: normalized intensity. Scale bars: 3 µm. b, f Average variation of the fluorescence intensity as a function of the STED power, together with the respective k and B values retrieved from the fitting of Eq. (6) to the data. c, g Phasor plot of the data shown in a and e, respectively. d, h SPLIT image components Fin, Fout, and Fbkgd, and the corresponding phase image ϕ. The white arrowheads point at pairs of foci that are better resolved in the SPLIT images. Color scale: phase ϕ (0–0.75). Scale bars: 1 µm