Fig. 5.

PbVac-mediated protection against Pf challenge. a Diagram of the experimental protocol. b Pf sporozoite infection of HC-04 human immortalized hepatocyte cultures incubated with purified IgG from mock- (grey), PbWT- (yellow) or PbVac (purple)-immunized rabbits. c qRT-PCR quantification of Pf hepatic infection inhibition in liver-humanized FRG mice following passive transfer of purified IgGs from mock (grey)-, PbWT (orange)- or PbVac (purple)-immunized rabbits and challenge by the bites of 20 Pf-infected mosquitoes (n = 3 mice per group). Measurements were taken from distinct samples. The boxes correspond to the 25th and 75th percentiles in B), and the minimum and maximum data range in C); the lines and bars indicate mean of infection and standard error of the mean, respectively; *p < 0.05; **p < 0.01, as determined by one-way ANOVA, corrected with Dunnett’s multiple comparisons test