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. 2018 Aug 24;8:12794. doi: 10.1038/s41598-018-30787-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of the intracellular expression of transcription factor GATA6 in human peritoneal monocytes/macrophages. Representative FACS dot-plots of the gating strategy for blood monocytes subsets based on CD14/CD16 expression and the level of intracellular expression of transcription factor GATA6 in blood monocytes are displayed (a). Representative FACS histograms of intracellular expression of transcription factor GATA6 in the three blood monocytes subsets are shown partially overlaid (b). Representative FACS dot-plots of the gating strategy of peritoneal subsets based on CD14/CD16 expression, and the level of intracellular expression of transcription factor GATA6 in peritoneal monocytes/macrophages are also displayed (c). Representative FACS histograms partially overlaid corresponding to GATA6 intracellular expression in the three different peritoneal monocytes/macrophages subsets are shown (d). Percentages of GATA6 positive cells present in peritoneum, indicated as mean ± SEM, are shown next to the corresponding histogram. Percentages referred to the totality (100%) of monocyte/macrophages into the correspondent subset. Empty histograms correspond to FMO control-PE and coloured histograms to GATA6-PE in blood (pink: CD14⁺⁺CD16⁻ classic subset; orange: CD14⁺⁺CD16⁺ intermediate subset; dark red: CD14^+/lowCD16⁺ non-classical subset) or peritoneum (light blue: CD14⁺⁺CD16⁻ subset; bright blue: CD14⁺⁺CD16⁺ subset; dark blue: CD14^highCD16^high subset). Correlation analysis of GATA6 intensity expression (MFI) related to the corresponding MFI of CD14 (black circles) and CD16 (black squares) for each monocyte/macrophage subset is displayed (e).