Table 3.
Markers | Total CD14+ cells | CD14/CD16 expression subsets | ||||||
---|---|---|---|---|---|---|---|---|
Blood | Peritoneum | Blood monocytes | pMo/Mφ | |||||
CD14++CD16− | CD14++CD16+ | CD14+/lowCD16+ | CD14++CD16− | CD14++CD16+ | CD14highCD16high | |||
CD11b+ | 158.6 ± 31.2 | 135.9 ± 36.1 | 133.9 ± 12.5 | 128.1 ± 17.6 | 59.7 ± 7.6 | 67.2 ± 15.6 * | 142.8 ± 28.6 | 303.1 ± 49.4 |
CD11c+ | 8.3 ± 1.4 | 64.5 ± 8.9*** | 8.6 ± 2.2 | 22.9 ± 6.6 | 23.8 ± 8.6 | 67.8 ± 12.6*** | 58.11 ± 8.5*** | 95.5 ± 12.8 |
CD40+ | 3.5 ± 0.5 | 14.7 ± 4.1** | 3.5 ± 0.9 | 4.3 ± 1.0 | 4.0 ± 1.0 | 6.0 ± 1.5 | 18.2 ± 3.6*** | 57.0 ± 12.2 |
CD62L+ | 29.9 ± 3.7 | 6.7 ± 1.4*** | 31.2 ± 3.7 | 15.9 ± 2.7 | 9.9 ± 1.7 | 3.1 ± 0.6*** | 7.3 ± 1.4* | 22.5 ± 5.9 |
CD64+ | 30.2 ± 2.6 | 28.7 ± 3.7 | 31.7 ± 2.8 | 22.1 ± 2.0 | 5.7 ± 0.6 | 16.6 ± 1.8*** | 30.6 ± 3.7* | 84.2 ± 12.5 |
CD80+ | 2.5 ± 0.6 | 7.0 ± 1.7** | 2.7 ± 0.5 | 3.0 ± 0.6 | 3.2 ± 0.5 | 2.8 ± 0.3 | 8.2 ± 1.6** | 22.1 ± 6.6 |
CD86+ | 16.4 ± 1.6 | 44.1 ± 11.0** | 15.4 ± 1.4 | 23.9 ± 2.4 | 26.3 ± 2.3 | 13.7 ± 1.5 | 57.7 ± 9.8*** | 162.0 ± 27.1 |
CD116+ | 45.6 ± 2.8 | 67.8 ± 11.5* | 47.2 ± 3.1 | 40.7 ± 4.7 | 19.8 ± 2.8 | 44.5 ± 5.0 | 72.2 ± 9.9** | 161.3 ± 25.9 |
CD119+ | 16.2 ± 2.3 | 48.7 ± 8.11*** | 13.1 ± 2.7 | 21.7 ± 5.4 | 20.2 ± 2.4 | 24.1 ± 2.3 | 62.2 ± 8.7** | 132.6 ± 29.6 |
CD206+ | 2.9 ± 0.4 | 23.0 ± 4.3*** | 2.9 ± 0.4 | 3.9 ± 0.5 | 4.4 ± 0.7 | 10.1 ± 1.1*** | 25.1 ± 4.2*** | 123.7 ± 22.5 |
HLA-DR+ | 18.7 ± 3.9 | 84.4 ± 16.0*** | 16.7 ± 3.4 | 60.0 ± 21.0 | 52.8 ± 9.6 | 55.6 ± 10.0*** | 113.1 ± 25.9 | 238.8 ± 51.6 |
Slan+ | 8.4 ± 1.9 | 44.8 ± 8.0*** | 8.4 ± 1.9 | 8.7 ± 1.9 | 6.6 ± 1.0 | 29.9 ± 0.1*** | 30.8 ± 0.8*** | 33.3 ± 1.4 |
Data represent the Mean ± SEM of median fluorescence intensity (MFI) for each membrane marker analyzed. *p < 0.05, **p < 0.01, and ***p < 0.001, ratios of positive monocyte/macrophages from peritoneum compared to monocytes from blood of healthy subjects.