Fig. 2.

PRR7 controls excitatory synapse numbers. a Representative immunofluorescent images of PSD-95 in hippocampal neurons transfected with GFP, HA-PRR7, or PRR7-GFP. White boxes indicate areas shown in enlarged Nxf images. b Quantitation of average PSD-95 puncta densities. n = 18–24 neurons per condition. c Representative immunofluorescent images of vGLUT1 in hippocampal neurons transfected with GFP, HA-PRR7, or PRR7-GFP. d Quantitation of average vGLUT1 puncta densities. n = 32–35. e Representative immunofluorescent images of PSD-95 and vGLUT1 in hippocampal neurons transfected with PRR7 RNAi. f Quantitation of PRR7 knockdown effect on PSD-95 and vGLUT1 puncta densities. n = 18–22. g, h Loss of PRR7 RNAi effect by shRNA-resistant PRR7 (HA-PRR7r). Representative images (g) and quantification of the data (h). Ctrl control. n = 30. i, j The effect of PRR7 or PRR7-GFP overexpression on dendritic spine densities in transfected neurons. Representative images (i) and quantification of the data (j). n = 18. Scale bars, 5 μm. Data are mean ± SEM. Two-way (b, d, f, h) or one-way ANOVA (j), post hoc Tukey’s test: F_2,124 = 207.6 (b), F_2,194 = 148.1 (d), F_1,78 = 76.06 (f), F_3,232 = 380.7 (h), and F_2,82 = 100.4 (j). ***P < 0.0001