Fig. 6.

Exosomal secretion of PRR7 is required for the elimination of excitatory synapses in neighboring neurons. a Blockade of exosomal secretion of HA-PRR7 by Rab27b DN. b–d Effect of Rab11a and Rab27 WT and DN on the PRR7-induced loss of PSD-95 clusters. Representative immunofluorescent images of PSD-95 in hippocampal neurons transfected with the constructs indicated above images (b). Quantitation of the data on PSD-95 cluster density (c) and intensity (d). n = 14 per condition. e, f Effect of APV and CNQX on the PRR7-induced loss of PSD-95 clusters. Representative immunofluorescent images of PSD-95 (e) and quantitation of the data (f). n = 15 each. Scale bars, 5 μm. Two-way ANOVA, post hoc Tukey’s test: F_5,156 = 278.4 (c), F_5,156 = 45.49 (d), F_5,168 = 199.7 (f). ***P < 0.0001 and *P = 0.0197. Data are mean ± SEM. Mr relative molecular weight