Fig. 4.

Reduced synaptic levels of proteins that mediate secretion in Vti1a/b-deficient neurons. a–d Representative examples of single DIV-14 neurons immunostained for Synaptophysin-1 as synaptic marker and Syntaxin-1 and SNAP25 (a), Munc18-1 and Munc13-1 (b), RIM1/2 and Bassoon (c), and Synaptobrevin-2 and Synaptophysin-1 (d). e–l Intensity distribution of the levels of Syntaxin-1 (e), SNAP25 (f), Munc13-1 (g), Munc18-1 (h), Bassoon (i), RIM1/2 (j), Synaptotagmin-1 (k), Synaptobrevin-2 (l) in all synapses (n = Over 15,000 synapses per protein and group). Statistical significance tested in Supplementary Figure 5. Scale bar = 2 µm