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. 2018 Aug 24;8:12780. doi: 10.1038/s41598-018-29448-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HNF-1A transactivation of a rat albumin promoter is repressed by SUMO-3 and PIASγ. (a,b) Transactivation analysis of HNF-1A in MIN6 cells transiently transfected with WT HNF-1A or mutants K205R, K273R, K506R, K205RK273R and K205RK273RK506R in the presence/absence of plasmids encoding SUMO-3, and/or PIASγ, as indicated in the figure, and together with the reporter plasmids pGL3-RA and pRL-SV40. Firefly luciferase activity was normalized to Renilla activity (control reporter) and empty vector was used as negative control. Each bar (a,b) represents the mean of nine readings ± SD; three parallel readings were conducted on each of three experimental days (n = 3). Measurements are given in fold activity relative to WT. *indicates p < 0.05, **indicates p < 0.001. EV = empty vector.