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. 2018 Aug 24;8:12780. doi: 10.1038/s41598-018-29448-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PIASγ does not influence the DNA binding ability of HNF-1A. (a) The DNA-binding ability of HNF-1A was assessed in nuclear fractions isolated from HeLa cells transiently transfected with WT HNF-1A or empty vector, and co-transfected with SUMO-3 and PIASγ. HNF-1A-DNA binding was analyzed by electrophoretic mobility shift assay (EMSA) after HNF-1A incubation with a Cy5 labeled DNA oligo (corresponding to HNF-1 binding site in the rat albumin). Bound complexes were separated on a 6% DNA retardation gel and the fluorescence signal was detected at 670 nm. Full-length gel is presented in Supplementary Fig. S6. (b) Quantification of binding by densiometric analysis is presented relative to WT alone (set to 100%). Each bar represents a mean of three independent experiments ±SD (n = 3).