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(a) Location of fatty acid (FFA) binding sites FA1-7 on human serum albumin (PDB: 1E7H), complexed with hexadecanoic (palmitic) acid [82]. High (magenta) and low (green) affinity sites are shown. (b) Location of site A, the multi-metal binding site (PDB: 5IJF), occupied by Zn2+ (blue) [57]. Site A and FA2 are both located between subdomains IA-IIA. The inter-domain nature and the proximity of FA2 to site A allows for the allosteric switching of metal ion binding [21].
