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. 2018 Aug 10;175(18):3685–3698. doi: 10.1111/bph.14433

Figure 2.

Figure 2

β3‐adrenoceptor activation contributes to the PVAT anticontractile effect. (A) Representative Western blot indicating expression of β3‐adrenoceptors within rat mesenteric PVAT. Three independent samples were analysed from each rat on one membrane. Incubation of intact vessels with PVAT with 10 μmol·L−1 CL‐316243 for 30 min did not alter the response to (B) noradrenaline (n = 5) but enhanced the anticontractile effect of (C) phenylephrine (n = 5). (D) Incubation of vessels with endothelium but lacking PVAT with 10 μmol·L−1 CL‐316243 did not alter the contractile response to phenylephrine (n = 5). Incubation of mesenteric vessels with PVAT with the β3‐adrenoceptor antagonist, 1 μmol·L−1 SR 59230A for 30 min potentiated constriction to (E) noradrenaline (n = 7) but had no effect on the contractile response to (F) phenylephrine (P = NS, n = 7). Data are expressed as mean ± SEM. *P < 0.05, two‐way ANOVA.