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. 2017 Aug 30;97(3):400–412. doi: 10.1093/biolre/iox108

Figure 4.

Figure 4.

MAPK7 regulates a subset of WNK1-regulated genes and promotes HESC migration and proliferation. (A) Western blot for total (MAPK7) and phosphorylated (pMAPK7) MAPK7 proteins. Quantification of MAPK7 phosphorylation is listed above the pMAPK7 band, expressed as the ratio of pMAPK7 to total MAPK7, normalized to beta-actin and relative to Veh siNT-treated cells. (B) Relative expression of MAPK7 mRNA. (C) Western blot for MAPK7 protein. (D) Relative mRNA expression of IGFBP1, PRL, CCL8, IL1B, IL15, EGR2, SMAD3, ITGA2, ITGA4, and ITGB3. Bars indicate mean values and SEM. Different letters denote means that are significantly different (P < 0.05, ANOVA with Tukey's MCA). (E) Cell migration into the scratch area at 0 and 24 h. (F) Quantification of distance migrated into the scratch area relative to EPC siNT-treated cells. Bars indicate mean values and SEM. *** P < 0.001, two-tailed t-test. (G) Relative proliferation of HESCs measured by the MTS assay. Points indicate mean values and SEM. ** P < 0.01, *** P < 0.001, two-tailed t-test.