Figure 1.
Ectopic BASL Reveals a Polarity Field Independent of Stomatal Lineages
(A) Induced 35S::GFP-BASL in speechless leaf stained with propidium iodide (PI). Scale bar is 50 μm in right panel.
(B) BASL vectors (orange arrow) assigned from BASL crescent to cell centroid. α between midline vector (black arrow) and BASL vector.
(C–E) Ectopic BASL vectors colored according to color wheel (in C) indicating α orientation in speechless leaves of (C) 50–200 μm, (D) 200–400 μm, and (E) 400–800 μm width categories. Leaf outlines shown.
(F–H) Vector orientation in speechless leaves pooled from widths (F) 50–200 μm (n = 185 cells, 4 leaves, σ = 55.34), (G) 200–400 μm (n = 1199 cells, 12 leaves, σ = 49.43), and (H) 400–800 μm (n = 2063 cells, 9 leaves, σ = 44.68). 0° represents proximodistal vector. Scale bars are 100 μm except for the right panel of (A).
See also Figure S1.