Figure 4.

Ectopic mCherry-BASL Localizes to the Opposite End of Cells to PIN1 Mirroring Convergence and Divergence Points at Serrations

(A) Induced 35S::mCherry-BASL in leaf primordium. Arrows indicate manually assigned BASL polarity based on curvature of the BASL crescent.

(B) PIN1::PIN1-GFP in same primordium as (A).

(C) mCherry-BASL and PIN1-GFP signals combined. Yellow box indicates magnified region of leaf. Scale bars are 20 μm in (A)–(C) and 10 μm in close-up regions of (C).

(D) Induced 35S::mCherry-BASL at serration of leaf 5. Arrows are manually assigned, and yellow arrows highlight cells in which BASL is not proximally localized.

(E) PIN1::PIN1-GFP in same serration as shown in (D).

(F) mCherry-BASL and PIN1-GFP signals combined. Projections allow visualization of margin cells. Scale bars are 50 μm in (D)–(F).

(G–I) Magnified regions of serration in (F) in blue (G), yellow (H), and magenta (I) boxes, respectively. z slices were selected to allow visualization of cells due to curvature of serrations. 35S::mCherry-BASL (left), PIN1::PIN1-GFP (middle), and combined signals (right). White dotted lines indicate leaf outline. Scale bars are 10 μm in (G)–(I).

See also Figure S3.