REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, Peptides, and Recombinant Proteins | ||
Propidium Iodide | Sigma-Aldrich | Cat# P4170 |
Spe I | Sigma-Aldrich | 11008943001 |
BspEI | New England Biolabs | Cat# R0540S |
ccdB-resistant one-shot E. coli | ThermoFisher Scientific | Cat# A10460 |
Difco agar | Becton & Dickinson | Cat# 214030 |
Oryzalin | Sigma-Aldrich | Cat# 36182 |
N-1-naphthylphthalamic acid (NPA) | ChemService | N-12507 |
Critical Commercial Assays | ||
iDNA genetics copy number analysis | iDNA genetics | N/A |
Experimental Models: Organisms/Strains | ||
Heat-shock inducible BASL (35S::GFP-BASL) | This paper | N/A |
BASL::GFP-BASL | [11] | N/A |
RFP-plasma membrane (pm-rb) | [47] | CD3-1008 |
RFP-plasma membrane with inducible 35S::GFP-BASL | This paper | N/A |
spch mutant (spch-1) | [48] | N/A |
spch with inducible BASL (35S::GFP-BASL) | This paper | N/A |
HS::Cre | [17] | N/A |
PIN1::PIN1-GFP | [49] | N/A |
Heat-shock inducible 35S::mCherry-BASL with PIN1::PIN1-GFP | This paper | N/A |
35S::TUA6-GFP | [50] | N/A |
Oligonucleotides | ||
F_BOB_lox_speI (GGGACTAGTATCGCGGCCGCTTCGAAA) | This paper | N/A |
R_BOB_lox_N (CTATACGAAGTTATACGCGTCTGT) | This paper | N/A |
R3_BOB_lox_EcoRV (GGGATATCATAACTTCGTATAAAGTAT CCTATACGAAGTTATACGCGTCTG) |
This paper | N/A |
Recombinant DNA | ||
pBOB vector | [51] | N/A |
TOPO4 vector | Invitrogen | N/A |
pB7WGC2 vector | [52] (VIB Gent) | N/A |
GFP-BASL entry clone | [11] | N/A |
Destination vector with lox-HDEL:CyPET:NOS-Terminator-lox (Active blue destination vector) | This paper | N/A |
Software and Algorithms | ||
cellfromleaves | This paper | Github; https://github.com/JIC-Image-Analysis/cells-from-leaves |
cellsfromleavestagger | This paper | Github; https://github.com/JIC-Image-Analysis/cells-from-leaves-tagger |
sampleArrows8 | This paper | Github; https://github.com/JIC-Image-Analysis/cells-from-leaves/tree/master/matlab_scripts |
cellLongAxisCorr7 | This paper | Github; https://github.com/JIC-Image-Analysis/cells-from-leaves/tree/master/matlab_scripts |
VolViewer | http://cmpdartsvr3.cmp.uea.ac.uk/wiki/BanghamLab/index.php/Software#Viewing_and_measuring_volume_images:_VolViewer |