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. 2018 Aug 20;28(16):2638–2646.e4. doi: 10.1016/j.cub.2018.06.019
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins

Propidium Iodide Sigma-Aldrich Cat# P4170
Spe I Sigma-Aldrich 11008943001
BspEI New England Biolabs Cat# R0540S
ccdB-resistant one-shot E. coli ThermoFisher Scientific Cat# A10460
Difco agar Becton & Dickinson Cat# 214030
Oryzalin Sigma-Aldrich Cat# 36182
N-1-naphthylphthalamic acid (NPA) ChemService N-12507

Critical Commercial Assays

iDNA genetics copy number analysis iDNA genetics N/A

Experimental Models: Organisms/Strains

Heat-shock inducible BASL (35S::GFP-BASL) This paper N/A
BASL::GFP-BASL [11] N/A
RFP-plasma membrane (pm-rb) [47] CD3-1008
RFP-plasma membrane with inducible 35S::GFP-BASL This paper N/A
spch mutant (spch-1) [48] N/A
spch with inducible BASL (35S::GFP-BASL) This paper N/A
HS::Cre [17] N/A
PIN1::PIN1-GFP [49] N/A
Heat-shock inducible 35S::mCherry-BASL with PIN1::PIN1-GFP This paper N/A
35S::TUA6-GFP [50] N/A

Oligonucleotides

F_BOB_lox_speI (GGGACTAGTATCGCGGCCGCTTCGAAA) This paper N/A
R_BOB_lox_N (CTATACGAAGTTATACGCGTCTGT) This paper N/A
R3_BOB_lox_EcoRV (GGGATATCATAACTTCGTATAAAGTAT
CCTATACGAAGTTATACGCGTCTG)
This paper N/A

Recombinant DNA

pBOB vector [51] N/A
TOPO4 vector Invitrogen N/A
pB7WGC2 vector [52] (VIB Gent) N/A
GFP-BASL entry clone [11] N/A
Destination vector with lox-HDEL:CyPET:NOS-Terminator-lox (Active blue destination vector) This paper N/A

Software and Algorithms

cellfromleaves This paper Github; https://github.com/JIC-Image-Analysis/cells-from-leaves
cellsfromleavestagger This paper Github; https://github.com/JIC-Image-Analysis/cells-from-leaves-tagger
sampleArrows8 This paper Github; https://github.com/JIC-Image-Analysis/cells-from-leaves/tree/master/matlab_scripts
cellLongAxisCorr7 This paper Github; https://github.com/JIC-Image-Analysis/cells-from-leaves/tree/master/matlab_scripts
VolViewer http://cmpdartsvr3.cmp.uea.ac.uk/wiki/BanghamLab/index.php/Software#Viewing_and_measuring_volume_images:_VolViewer