REAGENT or RESOURCE	SOURCE	IDENTIFIER
Deposited Data
20S Proteasome Core	This paper	EMDB: EMD-7946
26S Single-capped Proteasome	This paper	EMDB: EMD-7947
Fraction 4 proteomics data	This paper	PRIDE: PXD010026
Experimental Models: Cell Lines
HEK293T	ATCC	CRL3216
Software and Algorithms
Appion	(Lander et al., 2009)	http://nramm.nysbc.org/software/
FindEM	(Roseman, 2004)	N/A
DoG Picker	(Voss et al., 2009)	N/A
Proteome Discoverer	ThermoFisher Scientific	https://www.thermofisher.com/order/catalog/product/OPTON-30795
RELION	(Scheres, 2012)	http://www2.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page
CTFFIND4	(Rohou and Grigorieff, 2015)	http://grigoriefflab.janelia.org/ctf
cryoSPARC	(Punjani et al., 2017)	https://cryosparc.com/
Chimera	(Pettersen et al., 2004)	https://www.cgl.ucsf.edu/chimera/
SearchGUI	(Vaudel et al., 2011)	http://compomics.github.io/projects/searchgui.html
PeptideShaker	(Vaudel et al., 2015)	http://compomics.github.io/projects/peptide-shaker.html
Cytoscape	(Shannon et al., 2003)	http://www.cytoscape.org/
Other
Formvar/Carbon 400 mesh, Copper approx. grid hole size: 42μm	Ted Pella	01754-F