Fig. 3.

Ectopic expression of Not3 suppresses tumor formation in eyeful flies. a Qualitative and quantitative representation of the defective eyes upon ectopic expression of Not3 on wild-type flies with reduced Not3 expression. Bars show the percentage of eyes screened: blue, rescued eyes; orange, not rescued eyes; *** p < 0.001. Numbers 1 and 2 on each genotype represented on the graph refer to different recombinant clones. Three independent crosses were analyzed. The mean number of eyes screened is shown on the graph (Y-axis). Microscopy images show eyes of adult flies from representative genotypes, scale bars are 200 μM. qPCR analyses showing the Not3 expression levels from each genotype. Each bar represents the expression value of a pool of 10 individuals. b Qualitative and quantitative representation of the hyperplastic eyes upon ectopic expression of Not3 on eyeful flies. Bars show the percentage of eyes screened: blue, eyes with no hyperplasia; light orange, eyes classified as score 1 (presence of 1 fold); dark orange, eyes classified as score 2 (presence of 2–4 folds); ** p < 0.01, *** p < 0.001. Three independent crosses were analyzed. The mean number of eyes screened is shown on the graph (Y-axis). Microscopy images show eyes of adult flies from representative genotypes. Scale bars are 200 μM. c Quantification of BrdU, pH3, and cleaved-DCP1a positive cells on the posterior part of L3 larval eye-antennal imaginal discs. Values for 10 individual discs were plotted. Representative confocal images of eye-antennal imaginal discs of the indicated genotypes. Green staining: BrdU, pH3, or cleaved-DCP1a positive cells; red staining: ELAV protein. Scale bars are 70 μM; ns, not significant; * p < 0.05; ** p < 0.01; ***p < 0.001