Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 25;11:108. doi: 10.1186/s13045-018-0650-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 4 — The Not3 Not-box domain is important for normal protein functionality. a Qualitative and quantitative representation of the tumor burden upon downregulation of different CCR4-NOT complex subunits on the sensitized background. Bars show the percentage of eyes screened: blue, normal eyes; orange, hyperplastic eyes; ** p < 0.01; *** p < 0.001. The mean number of eyes screened is shown on the graph (Y-axis) and three independent crosses were established. Microcopy images show the eyes of adult flies from representative genotypes. Scale bars are 200 μM. Black arrow indicates the presence of metastasis. b Quantitative representation of the whole eye area (region with presence of differentiated photoreceptors) on representative adult eyes (n = 8) with downregulation of Not3 or twin on the sensitized genetic background. Control eyes measured values were considered as 100%; ns, not significant; ** p < 0.01; *** p < 0.001. c Schematic representation of the human CNOT3 and the fly Not3 proteins. ClustalW2 alignment analyses show that the coiled-coil (green) and Not-box (orange) regions are present in both species. Designed UAS constructs are also shown: UAS-Not3 (wild-type Not3), UAS-Not3 ΔCC (deletion of the coiled-coil domain region), UAS-Not3 ΔNB (deletion of the Not-box domain). d Qualitative and quantitative representation of the defective eyes upon ectopic expression of Not3, Not3-ΔCC, and Not3-ΔNB on wild-type flies with reduced Not3 expression. The mean number of eyes screened is shown on the graph (Y-axis), and three independent crosses were established. Bars show the percentage of eyes screened: blue, normal eyes; light orange, eyes classified as “almost rescued”; dark orange, eyes classified as “not rescued”; *** p < 0.001. In green color, comparisons to UAS-GFP control flies; black color, comparison to UAS-LacZ control flies. Microscopy images show the eyes of adult flies from each category. Scale bars are 200 μM. e Qualitative and quantitative representation of the defective eyes upon ectopic expression of Not3, Not3-ΔCC, and Not3-ΔNB on eyeful flies. Bars show the percentage of eyes screened: blue, normal eyes; light orange, eyes classified as score 1 (presence of 1 fold); dark orange, eyes classified as score 2 (presence of 2–4 folds); ns, means not significant, ** p > 0.01, *** p < 0.001. The mean number of eyes screened is shown on the graph (Y-axis). In green color, comparisons to UAS-GFP control flies; black color, comparison to UAS-LacZ control flies. Microscopy images show the eyes of adult flies from each category. Scale bars are 200 μM. f Quantitative representation of the whole eye area (region with presence of differentiated photoreceptors) on representative adult eyes (n = 8) with ectopic expression of Not3 wild-type or Not3 mutants on the eyeful genetic background. Control eyes measured values were considered as 100%; ns, not significant; * p < 0.05; ** p < 0.01