Table 2.
Protocol | Peptide | Protein | Peptide/protein | ||||
---|---|---|---|---|---|---|---|
Mean ± SD | ID ≥ 2 | Percentage quantified | Mean ± SD | ID ≥ 2 | Percentage quantified | ||
FASP | 6576 ± 237a | 6395 | 86.4 ± 0.7a | 1745 ± 71a | 1725 | 95.2 ± 0.7a | 3.8 ± 0.1ab |
SDS-ISD | 4584 ± 110b | 4513 | 86.1 ± 1.9a | 1326 ± 81b | 1290 | 95.5 ± 0.2a | 3.5 ± 0.2b |
SDC-FASP | 6907 ± 219a | 6748 | 86.5 ± 1.0a | 1829 ± 98a | 1800 | 94.9 ± 0.3a | 3.8 ± 0.1ab |
SDC-ISD | 4938 ± 424b | 4733 | 86.4 ± 3.5a | 1378 ± 51b | 1352 | 95.1 ± 1.4a | 3.6 ± 0.2b |
OP-ISD | 5909 ± 207c | 5628 | 84.5 ± 1,6a | 1490 ± 65b | 1460 | 94.4 ± 0.2a | 4.0 ± 0.1a |
Peptides and proteins were identified and quantified using label-free proteomics
ID ≥ 2, peptides or proteins identified in at least two replicates; percentage quantified, percentage of peptides or proteins quantified from the identified ones in at least two replicates. Different lowercase letters indicate significant difference at 0.05 level. Data are mean ± SD (n = 3)