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. 2018 Aug 25;17:200. doi: 10.1186/s12944-018-0849-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — PCR-based genotyping analysis, confirmed by gel electrophoresis. a Curves of fluorescence (F) versus temperature (T) for sequence-specific base-quenched probe complementary to the SR-BI knockout gene sequence. b Derivative melting curves (-dF/dT vs. T) that depict the same data shown in panel A, wild-type (SR-BI^+/+), heterozygous (SR-BI^+/−), and homozygous (SR-BI^−/−) mutant mice. c Two sets of primer pairs specific for the wild-type (primers 2 and 3) or targeted mutant (primers 2 and 4) alleles were used to screen genomic DNA by PCR as described. Representative results from wild-type (SR-BI^+/+, lanes 3 and 4), heterozygous (SR-BI^+/−, lanes 5 and 6), and homozygous mutant (SR-BI^−/−, lanes 1 and 2) animals are shown