Table 2.
Sequences of primers and probes for real-time RT-PCR
| Gene | Primer / Probe | Sequence (5’ to 3’) |
|---|---|---|
| Mouse ApoM | Forward | GCTTTCTCCTCTACAATCGGTCAC |
| Reverse | CGGGCAGGCCTCTTGATT | |
| Probe | FAM- ACCTCTTGCTTGGACTTCAAAGCCTTCTTA-TAMRA | |
| Mouse ApoAI | Forward | CAGTTTGAATCCTCCTCCTTGG |
| Reverse | GGTTATCCCAGAAGTCCCGAG | |
| Probe | FAM-CAACAGCTGAACCTGAATCTCCTGGAA-TAMRA | |
| Mouse GAPDH | Forward | TCTTGTGCAGTGCCAGCCT |
| Reverse | TGAGGTCAATGAAGGGGTCG | |
| Probe | FAM-AGGTCGGTGTGAACGGATTTGGC-TAMRA | |
| Human ApoM | Forward | CTGACAACTCTGGGCGTGGAT |
| Reverse | TGTCCACAGGGTCAAAAGTTGC | |
| Probe | FAM-AGTTCCCAGAGGTCCACTTGGGCCA-BHQ1 | |
| Human GAPDH | Forward | CAGGGCTGCTTTTAACTCTGGT |
| Reverse | CATGGGTGGAATCATATTGGAAC | |
| Probe | CY5-TGGATATTGTTGCCATCAATGACCCCT-BHQ2 |