Fig. 1.
JEV infection stimulates the activity of NMDAR. a N2a cells and c–d primary mouse neuron/glia cultures were infected with JEV at an MOI of 1, and samples were collected at indicated time points. b Mice were inoculated intraperitoneally with DMEM or 106 PFU JEV, and brain tissues were collected and lysed on day 6 post-infection. The extracts from cultured cells and mice brain tissues were either subjected to Western blot analysis (a–c) or to flow cytometry analysis (d). The lower panels represent the quantification of data corresponding to a–d. Protein levels were quantified by immunoblot scanning and normalized to the amount of β-tubulin. The relative intracellular calcium concentration was representative with mean fluorescence intensity (MFI) and normalized to the control. Data are expressed as means ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001