Fig. 5.

NMDAR blockade by MK-801 suppresses JEV-triggered glutamate release and inflammatory response in mouse brain. Mice were subjected to JEV infection and MK-801 treatment paradigm as described in Fig. 4a. Brain samples were collected on day 5 after JEV infection. a, b The mRNA (a) and protein (b) levels of TNF-α, CCL-5, IL-1β, and CCL-2 in brain lysates were analyzed by quantitative real-time PCR and ELISA, respectively. c The glutamate protein levels in brain lysates were determined by ELISA. Similar results were obtained in three mice. *P < 0.05, **P < 0.01