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. 2018 Aug 8;2018:4180857. doi: 10.1155/2018/4180857

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Copyright © 2018 Shuhui Zheng et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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TβRIII RNAi enhanced TGF-β3-induced chondrogenic differentiation of hMSCs. SiNC- or SiTβRIII-infected cells were cultured in control medium (C-SiNC group; C-SiTβRIII group) or chondrogenic medium containing TGF-β3 (T-SiNC group; T-SiTβRIII group) for 14 days. (a) Upper panels show glycosaminoglycan (GAG) expression by Alcian blue staining; lower panels show COL II expression by immunohistochemistry. Scale bar = 100 μm. (b) GAG content was quantitatively analyzed and normalized by DNA content. (c) Real-time PCR analysis of COL2A1 and SOX9 mRNA levels in hMSCs from different groups. Error bars represent the means ± SD, n = 3. ^∗P < 0.05 versus C-SiNC group; ^#P < 0.05 versus T-SiNC group.