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IFNγ and TNFα costimulation is cytotoxic to confluent MC3T3-E1 monolayers. Cells were seeded in 96-well plates, incubated for 5 days to form a confluent monolayer, and treated with 10 ng/ml IFNγ and/or 5 ng/ml TNFα. Cell viability was monitored over time. Data represent the mean ± SEM of three independent experiments, with the percentage of cytotoxicity indicated.
