Figure 1.
Migration of human B cells across simulated retinal endothelium. B cell isolates from individual adult human subjects were migrated across a human retinal endothelial cell monolayer in a transwell for 18 hours (0.5 × 106 cells/transwell). Blocking antibody directed against ICAM-1 [A & C] or blocking antibody directed against VCAM-1 [B & D] – or isotype-matched negative control IgG – was added to the upper chambers of the transwells prior to addition of the B cell isolates. In graphs [A] and [B], each pair of circles joined by a line represents the mean number of migrated B cells in the presence of control antibody or blocking antibody for individual human subjects. n = 3 transwells/condition. Data were analyzed by one-tailed paired Student’s t-test. In graphs [C] and [D], bars represent mean number of migrated B cells – with error bars showing standard error of the mean – in the presence of control antibody or blocking antibody for numbered individual human subjects. n = 3 transwells/condition. Data were analyzed by one-tailed unpaired Student’s t-test. *=p≤0.05; **=p⩽0.01; ***=p⩽0.001.