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. 2018 Aug 20;9:1809. doi: 10.3389/fimmu.2018.01809

Figure 2.

Figure 2

Specificity of FcγRII forms for human IgG subclasses. FcγR expression and IgG binding to IIA1.6 cells expressing macaque mFcγRIIa3-H131 or mFcγRIIa1-H131 and human allelomorphs FcγRIIa3-R131 FcγRIIa1-R131, FcγRIIa1-H131 expressed on IIA1.6 cells. (A) Expression levels of receptor (heavy black open histogram) determined using a rabbit polyclonal anti-FcγRIIa ectodomain antiserum (pAb) (1:100) and PE conjugated anti-rabbit IgG secondary antibody. Background binding of pAb to untransduced IIA1.6 cells (gray filled histogram). (B) Binding of human IgG subclass-IgG1, IgG2, IgG3, and IgG4 (10 µg/µL) complexed with PE conjugated human anti-F(ab′)2 (5 µg/µL) (heavy black open histogram). Background binding of immune complexes to untransduced IIA1.6 cells (gray filled histogram). For full titration curve see Figure S1 in Supplementary Material.