Figure 6.

Increased levels of β-hexosaminidase release. RBL cells expressing human allelomorphs FcγRIIa1-R¹³¹ (▾) or FcγRIIa3 (●) as indicated. Transfectants were stimulated with (A) anti-FcγRIIa mAb 8.2 agonist (100–13.35 µg/mL) and (B) IgE anti-TNP/TNP:BSA (0.1–400 ng/mL) for 30 min at 37°C. Supernatant was then incubated with β-hexosaminidase substrate (4 mM p-NAG) and the percentage normalized cell total OD plotted as a dose response curve (mean ± SEM, n = 3). Student t-test, p-value = 0.0005.