Table 2.
Hypothalamic and brain stem p-STAT3 and SOCS3 in experiments 2 and 3
|
Experiment 2: Fourth-Ventricle Leptin Infusion |
|||||
|---|---|---|---|---|---|
| Saline | 0.01 μg Leptin | 0.1 μg Leptin | 0.3 μg Leptin | 0.6 μg Leptin | |
| Hypothalamic p-STAT3/total STAT3 | 0.63 ± 0.16 | 0.63 ± 0.08 | 1.85 ± 0.92 | 1.22 ± 0.37 | 1.64 ± 0.44 |
| Hypothalamic SOCS3/actin | 3.2 ± 0.4 | 4.2 ± 0.6 | 3.3 ± 1.1 | 3.7 ± 0.4 | 3.1 ± 0.5 |
| BS p-STAT3/total STAT3 | 0.44 ± 0.15 | 0.80 ± 0.10 | 0.62 ± 0.19 | 0.54 ± 0.13 | 0.53 ± 0.11 |
| BS SOCS3/actin | 0.15 ± 0.06 | 0.19 ± 0.05 | 0.23 ± 0.03 | 0.22 ± 0.02 | 0.20 ± 0.02 |
| Experiment 3: Third-Ventricle Leptin Infusion | |||||
| Saline | 0.005 μg Leptin | 0.01 μg Leptin | 0.05 μg Leptin | 0.1 μg Leptin | |
| Hypothalamic p-STAT3/total STAT3 | 0.52 ± 0.15 | 0.45 ± 0.09 | 0.90 ± 0.11 | 1.01 ± 0.23 | 0.99 ± 0.17 |
| Hypothalamic SOCS3/actin | 0.47 ± 0.18a | 0.26 ± 0.11a,b | 0.15 ± 0.12a,b | 0.07 ± 0.01b | 0.04 ± 0.01b |
| BS p-STAT3/total STAT3 | 0.64 ± 0.13 | 0.74 ± 0.19 | 0.62 ± 0.16 | 0.53 ± 0.13 | 0.42 ± 0.10 |
| BS SOCS3/actin | 0.52 ± 0.13 | 0.51 ± 0.12 | 0.51 ± 0.07 | 0.64 ± 0.04 | 0.73 ± 0.12 |
Values are means ± SE for groups of 7–9 rats euthanized after 16 days of third- or fourth-ventricle infusions of different doses of leptin in experiments 2 and 3. Data are arbitrary units from Western blot analysis of tissue samples and cannot be directly compared between experiments or between hypothalamus and brain stem because samples from each brain area for each experiment were run at different times. BS, brain stem. Values for a specific measure that do not share a common superscripted letter are significantly different at P < 0.05.