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. 2018 Aug 20;11:4999–5007. doi: 10.2147/OTT.S167053

Figure 4.

Figure 4

lncRNA MEG3 negatively regulates Rac1 gene expression.

Notes: (A) Diagram of the binding sequence of MEG3-RAC1 by blast.ncbi.nlm.nih.gov prediction. (B) MEG3 inhibited the expression of Rac1 at the mRNA level in both Hela and SiHa cells. (C) MEG3 inhibited the expression of Rac1 at the protein level in both Hela and SiHa cells. (D) qRT-PCR analysis of expression of lncRNA Rac1 expression in NCE, CIN, and SCC tissue samples (n=20). ***P<0.001. (E) Analysis of correlation of lncRNA MEG3 and Rac1 expression in SCC tissue samples (n=20) by linear regression analysis. (F) Expression of Rac1 protein level was detected by immunoblotting in non-tumoral HcerEpic and human cervical carcinoma cells (C-33A, C4-1, Caski, SiHa, and Hela). (G) Analysis of correlation of lncRNA MEG3 and Rac1 expression in non-tumoral HcerEpic and human cervical carcinoma cells (C-33A, C4-1, Caski, SiHa, and Hela) by linear regression analysis. *P<0.05, **P<0.01.

Abbreviations: con, control; NCE, normal cervical epithelium; CIN, cervical intraepithelial neoplasia; SCC, squamous cell carcinoma; HcerEpic, human cervical epithelial cells; lncRNA, long non-coding RNA; MEG3, maternally expressed gene 3; qRT-PCR, quantitative RT-PCR.