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. 2018 Aug 20;9:1936. doi: 10.3389/fmicb.2018.01936

FIGURE 4.

FIGURE 4

Analysis of ROP and ASP5, MYR1, and IST deletion mutants on the ability of T. gondii to inhibit NO production (nitrite in μM) of activated macrophages. (A) NO production of non-infected (Control) or activated J774-A1 cells infected with parental (RHΔku80 strain) or various ROP deletion strains of T. gondii at 24 h post-infection. Mean ± SEM (n = 3 experiments, each with 12 replicates). ∗∗∗∗P ≤ 0.0001, one-way ANOVA with Tukey post-test. (B) NO production of non-infected (Control) or activated J774-A1 cells infected with parental (RHΔku80 strain) or Δasp5, Δmyr1, or Δist mutant strains of T. gondii at 24 h post-infection. Mean ± SEM (n = 3 experiments, each with 12 replicates). ∗∗∗∗P ≤ 0.0001, one-way ANOVA with Tukey post-test. (C) NO production of non-infected (Control) or activated RAW 264.7 cells infected with parental (RHΔku80 strain) or various ROP deletion strains of T. gondii at 24 h post-infection. Mean ± SEM (n = 3 experiments, each with 12 replicates). ∗P ≤ 0.05, ∗∗P ≤ 0.01, ∗∗∗P ≤ 0.001, ∗∗∗∗P ≤ 0.0001, one-way ANOVA with Tukey post-test. (D) NO production of non-infected (Control) or activated RAW 264.7 cells infected with parental (RHΔku80 strain) or Δasp5, Δmyr1, and Δist mutant strains of T. gondii at 24 h post-infection. Mean ± SEM (n = 3 experiments, each with 12 replicates). ∗P ≤ 0.05, ∗∗P ≤ 0.01, one-way ANOVA with Tukey post-test.