Table 1.
Primer sequences for RT-PCR (mouse)
| Target Gene | Primer Sequence (5′→3′) | Target Gene | Primer Sequence (5′→3′) |
|---|---|---|---|
| mPGES-1 F | AGCACACTGCTGGTCATCAA | NLRP3 F | CGAGACCTCTGGGAAAAAGCT |
| mPGES-1 R | CTCCACATCTGGGTCACTCC | NLRP3 R | GCATACCATAGAGGAATGTGATGTACA |
| mPGES-2 F | GCTGGGGCTGTACCACAC | ASC F | ACTTCTGTGACCCTGGCAATGAG |
| mPGES-2 R | GATTCACCTCCACCACCTGA | ASC R | GCTGAGCAGCTGCAAACGAC |
| cPGES F | GGTAGAGACCGCCGGAGT | Caspase-1 F | GATGGCACATTTCCAGGACTGA |
| cPGES R | TCGTACCACTTTGCAGAAGCA | Caspase-1 R | TGTTGCAGATAATGAGGGCAAGAC |
| COX1 F | TAATCCCAGCACTTGGAAGG | IL-1β F | GAAATGCCACCTTTTGACAGTG |
| COX1 R | TGGCTGGCCTAGAACTCACT | IL-1β R | TGGATGCTCTCATCAGGACAG |
| COX2 F | TGGGTGTGAAGGGAAATAAGG | Fibronectin F | ATGTGGACCCCTCCTGATAGT |
| COX2 R | CATCATATTTGAGCCTTGGGG | Fibronectin R | GCCCAGTGATTTCAGCAAAGG |
| EP1 F | TAACGATGGTCACGCGATGG | α-SMA F | CCCAACTGGGACCACATGG |
| EP1 R | ATGCAGTAGTGGGCTTAGGG | α-SMA R | TACATGCGGGGGACATTGAAG |
| EP2 F | ATGCTCCTGCTGCTTATCGT | Collagen III F | CTGTAACATGGAAACTGGGGAAA |
| EP2 R | AGGGCCTCTTAGGCTACTGC | Collagen III R | CCATAGCTGAACTGAAAACCACC |
| EP3 F | TTGGGCTTGCTGGCTCTG | TGF-β F | GCAACATGTGGAACTCTACCAGA |
| EP3 R | CGTCTCCGTGGTGATTCTGC | TGF-β R | GACGTCAAAAGACAGCCACTCA |
| EP4 F | GTTCCGAGACAGCAAAAGC | GAPDH F | TGACCTCAACTACATGGTCTACA |
| EP4 R | CACCCCGAAGATGAACATCAC | GAPDH R | CTTCCCATTCTCGGCCTTG |
mPGES, microsomal prostaglandin E2 synthase; COX, cyclooxygenase; cPGES, cytosolic PGES; EP, prostaglandin E receptor; NLRP3, Nod-like receptor pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing a caspase recruitment domain; IL-1β, interleukin-1β; IL-18, interleukin-18; α-SMA, α-smooth muscle actin; TGF-β, transforming growth factor-β; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.