Fig. 4.

Complement factor D-deficient (FD^−/−) mice were more susceptible to oxidative stress. Wild-type (WT) and FD^−/− mice were allowed free access to ethanol [EtOH, 32%, day (d) 25] or pair-fed control diets. A: paraffin-embedded liver sections were deparaffinized, and 4-hydroxynonenal adducts were detected by immunohistochemistry. B: positive staining was quantified in ×10 images from at least three images per slide using Image-Pro Plus software. C: liver lysates were prepared and proteins were separated by SDS-PAGE. CYP2E1 and heat shock cognate 71 kDa protein (HSC70, loading control) were measured by Western blot. Relative expression is denoted as arbitrary units of density. Values represent means ± SE; values with different superscripts were significantly different from each other (P < 0.05); n = 4 pair-fed and 3–6 EtOH-fed mice.