Fig. 5.

Hepatic complement component 1, Q subcomponent (C1q) deposition was increased in wild-type (WT) and complement factor D-deficient (FD^−/−) mice after short-term and chronic ethanol (EtOH) feeding. WT and FD^−/− mice were allowed free access to EtOH [11%, day (d) 4 and 32%, day 25] or pair-fed control diets. A: frozen liver sections were immunostained for C1q deposition. Representative images for C1q deposition in pair-fed controls for chronic 25-day studies were similar to 4 days (data not shown). All images were acquired using a ×40 oil immersion objective. Positive immunofluorescence after short-term (B) and chronic (C) EtOH feeding was quantified from at least three images per slide using Image-Pro Plus software. Values represent means ± SE; values with different superscripts were significantly different from each other (P < 0.05); n = 4 pair-fed and 6 EtOH-fed mice.