Fig. 6.

Hepatic C3b deposition was impaired in complement factor D-deficient (FD^−/−) mice after chronic, but not short-term, ethanol (EtOH) feeding. WT, complement component 1, Q subcomponent-deficient (C1qa^−/−), complement protein 4-deficient (C4^−/−), FD^−/−, and C1qa/FD^−/− mice were allowed free access to EtOH [11%, day (d) 4 and 32%, day 25] or pair-fed control diets. A: frozen liver sections were immunostained for C3b/iC3b/C3c deposition. Representative images for C3b/iC3b/C3c deposition in pair-fed controls for chronic 25-day studies were similar to 4 days (data not shown). All images were acquired using a ×40 oil immersion objective. Positive immunofluorescence after short-term (B) and chronic (C) EtOH feeding was quantified for C4^−/−, FD^−/−, and C1qa/FD^−/− mice from at least three images per slide using Image-Pro Plus software. Positive immunofluorescence for C1qa^−/− has been previously reported (9). Values represent means ± SE; values with different superscripts were significantly different from each other (P < 0.05); n = 4–8 pair-fed and 5–12 EtOH-fed mice.