Table 7.
Results of nCounter validation
| IBS-C vs. HC | IBS-D vs. HC | IBS vs. HC | ||
|---|---|---|---|---|
| Gene | AveExpr (all n = 45) | FC (FDR) | FC (FDR) | FC (FDR) |
| AK9 | 11.7 | 1.1 (0.74) | 1.1 (0.63) | 1.1 (0.61) |
| ALDH3A1 | 11.1 | 0.7 (0.07)* | 0.8 (0.3) | 0.7 (0.06)* |
| ALDH9A1 | 14.8 | 1.1 (0.5) | 1.0 (0.79) | 1.0 (0.86) |
| AQP9 | 8.5 | 0.6 (0.17) | 0.5 (0.21) | 0.6 (0.06)* |
| CCDC134 | 11.9 | 1.0 (0.76) | 0.9 (0.54) | 0.9 (0.48) |
| CDC42EP5 | 16.7 | 1.1 (0.3) | 1.0 (0.67) | 1.1 (0.44) |
| CDK5 | 11.2 | 1.3 (0.05) | 1.1 (0.57) | 1.2 (0.09)* |
| CLDN15 | 12.5 | 1.0 (0.91) | 0.9 (0.61) | 1 (0.85) |
| CRHR1 | 6.4 | 0.5 (0.05) | 0.6 (0.41) | 0.5 (0.06)* |
| DBI | 16.2 | 1.1 (0.33) | 1.0 (1.0) | 1.0 (0.59) |
| DHRS12 | 12.4 | 0.9 (0.48) | 0.9 (0.61) | 0.9 (0.48) |
| EFR3B | 9.6 | 1.0 (0.87) | 0.9 (0.79) | 1.0 (0.93) |
| ELMOD3 | 12.3 | 0.8 (0.11)* | 0.9 (0.61) | 0.9 (0.18) |
| EZH2 | 13.0 | 1.0 (0.74) | 1.0 (0.83) | 1.0 (0.94) |
| FABP3 | 11.5 | 1.0 (0.94) | 1.0 (0.96) | 1.0 (0.97) |
| GABBR1 | 12.8 | 0.6 (0.01) | 0.8 (0.54) | 0.7 (0.04) |
| GABRA1 | 8.6 | 0.9 (0.76) | 1.0 (1.0) | 1.0 (0.88) |
| GABRG1 | 6.2 | 0.5 (0.05) | 0.6 (0.21) | 0.6 (0.04) |
| GH1 | 8.0 | 0.7 (0.12)* | 0.6 (0.21)* | 0.7 (0.06)* |
| HMGA1 | 14.3 | 1.0 (0.87) | 1.1 (0.57) | 1.1 (0.59) |
| HMGB1 | 17.0 | 1.2 (0.05) | 1.1 (0.3) | 1.2 (0.06)* |
| HTR3A | 7.9 | 0.8 (0.33) | 0.7 (0.21) | 0.7 (0.14)* |
| IL17RD | 9.9 | 0.8 (0.12) | 0.9 (0.54) | 0.8 (0.16) |
| IL17RE | 14.9 | 0.9 (0.29) | 1.0 (0.82) | 0.9 (0.48) |
| ITPKB | 12.6 | 0.8 (0.05) | 0.8 (0.21)* | 0.8 (0.04) |
| JRK | 11.4 | 0.9 (0.63) | 0.9 (0.61) | 0.9 (0.48) |
| KAT5 | 14.2 | 1.1 (0.59) | 1.1 (0.61) | 1.1 (0.48) |
| MLN | 6.5 | 1.1 (0.87) | 0.7 (0.45) | 0.9 (0.61) |
| MUC22 | 7.3 | 0.6 (0.05) | 0.7 (0.21) | 0.6 (0.04) |
| MUC4 | 15.9 | 0.7 (0.02) | 0.8 (0.41) | 0.8 (0.04) |
| MUCL1 | 6.4 | 0.8 (0.62) | 0.8 (0.61) | 0.8 (0.48) |
| NECTIN1 | 13.3 | 1.0 (0.76) | 1.0 (0.83) | 1.0 (0.97) |
| NKX2-1 | 7.1 | 0.6 (0.08) | 0.6 (0.21) | 0.6 (0.04) |
| NKX3-1 | 8.1 | 0.9 (0.48) | 0.9 (0.61) | 0.9 (0.48) |
| NOTCH4 | 10.8 | 0.9 (0.64) | 1.0 (0.99) | 1.0 (0.85) |
| NPBWR1 | 7.5 | 1.1 (0.74) | 0.9 (0.82) | 1.0 (0.97) |
| NRG3 | 8.3 | 0.9 (0.74) | 1.0 (0.82) | 0.9 (0.77) |
| OPHN1 | 12.5 | 1.1 (0.51) | 1.1 (0.61) | 1.1 (0.48) |
| OPLAH | 12.2 | 0.7 (0.11) | 0.8 (0.3) | 0.8 (0.09) |
| OPRM1 | 6.7 | 0.8 (0.62) | 0.7 (0.59) | 0.8 (0.48) |
| PALLD | 15.3 | 1.1 (0.62) | 1.1 (0.61) | 1.1 (0.51) |
| PCDHA3 | 8.5 | 0.6 (0.02) | 0.8 (0.29) | 0.7 (0.04) |
| PDE11A | 9.5 | 0.8 (0.32) | 0.9 (0.61) | 0.9 (0.36) |
| PDE2A | 10.5 | 0.7 (0.02) | 0.9 (0.61) | 0.8 (0.06)* |
| PPP1CA | 17.0 | 1.1 (0.18) | 1.2 (0.3) | 1.2 (0.13)* |
| PPP1R1A | 8.9 | 0.7 (0.16) | 0.8 (0.54) | 0.8 (0.18) |
| RAMP1 | 9.4 | 0.7 (0.09)* | 0.7 (0.21)* | 0.7 (0.04) |
| RBM5 | 14.9 | 0.9 (0.33) | 1.0 (0.61) | 0.9 (0.44) |
| RNF19A | 14.9 | 1.0 (0.76) | 1.0 (0.99) | 1.0 (0.86) |
| S100A1 | 7.7 | 0.8 (0.37) | 1.2 (0.61) | 1.0 (0.86) |
| SGSM2 | 13.0 | 0.8 (0.05) | 0.9 (0.62) | 0.8 (0.15) |
| SIGMAR1 | 13.5 | 1.0 (0.76) | 1.0 (0.82) | 1.0 (0.78) |
| SLC10A4 | 7.2 | 0.5 (0.02) | 0.6 (0.21) | 0.6 (0.03) |
| SLC17A3 | 8.4 | 1.0 (0.91) | 0.9 (0.61) | 1.0 (0.85) |
| SMARCA4 | 14.7 | 1.1 (0.24) | 1.1 (0.54) | 1.1 (0.22) |
| SOX5 | 9.9 | 0.7 (0.01) | 0.8 (0.21) | 0.7 (0.03) |
| SPATS2 | 12.6 | 1.2 (0.16) | 1.1 (0.57) | 1.1 (0.21) |
| SSPO | 6.2 | 0.5 (0.08)* | 0.5 (0.21) | 0.5 (0.04) |
| ST3GAL3 | 12.2 | 0.9 (0.32) | 0.9 (0.56) | 0.9 (0.29) |
| THBD | 11.7 | 0.7 (0.12) | 0.8 (0.54) | 0.8 (0.15) |
| TMEM44 | 13.7 | 0.9 (0.76) | 0.9 (0.72) | 0.9 (0.73) |
| TMEM80 | 13.6 | 1.2 (0.12)* | 1.0 (0.98) | 1.1 (0.44) |
| TRIM37 | 13.4 | 1.1 (0.51) | 1.1 (0.61) | 1.1 (0.48) |
| TXLNG | 13.3 | 0.9 (0.65) | 0.9 (0.61) | 0.9 (0.53) |
| UGT1A9 | 15.2 | 1.0 (0.78) | 1.0 (0.98) | 1.0 (0.87) |
| USP34 | 15.1 | 0.9 (0.09) | 1.0 (0.61) | 0.9 (0.2) |
| YIF1B | 12.9 | 1.1 (0.33) | 0.9 (0.61) | 1.0 (0.87) |
Boldface indicates FDR < 0.05. Unmarked values < 0.05 indicate direction did not match microarray. AveExpr, average expression (log2 transformed); FC, fold change, FDR, Benjamini-Hochberg adjusted P value.
*
Unadjusted P value < 0.05.