Table 8.
Comparison of findings from current study for genes found to be differentially expressed in the literature
| Fold Change, P Value |
|||||
|---|---|---|---|---|---|
| Symbol | Published Change & Subgroup (Ref.) | IBS | IBS-C | IBS-D | Product |
| ALDOB*** | ↑D (22) | 0.9 (0.3) | 0.8 (0.06) | 1.0 (0.88) | Fructose-bisphosphate aldolase B |
| ALDOC*** | ↑D (22) | 1.3 (0.05) | 1.6 (0.01) | 1.1 (0.45) | Fructose-bisphosphate aldolase C |
| AQP8 | ↓D (121) | 1.3 (0.25) | 1.7 (0.06) | 1.0 (0.94) | Aquaporin-8 |
| BIRC5 | ↑D (22) | 1.1 (0.53) | 1.5 (0.02) | 0.8 (0.16) | Baculoviral IAP repeat-containing protein 5 isof. 3 |
| C4BPA*** | ↑D (21) | 0.9 (0.29) | 0.7 (0.002) | 1.2 (0.13) | Complement component 4 binding protein, alpha |
| CACNA1H*** | ↑ (102) | 1.1 (0.57) | 1.2 (0.07) | 0.9 (0.36) | Voltage-dep. T-type calcium channel sub. Alpha-1h isof. A |
| CARD16*** | ↑ (2) | 1.1 (0.44) | 1.1 (0.25) | 1.0 (0.86) | Caspase recruitment domain-containing protein 16 isof. 1 |
| CASP1*** | ↓ (2) | 0.9 (0.55) | 1.0 (0.7) | 0.8 (0.16) | Caspase-1 isof. Gamma |
| CCL20*** | ↑D (21, 22) | 0.8 (0.12) | 0.6 (0.004) | 1.1 (0.7) | C-C motif chemokine 20 isof.1 precursor |
| CD163L1*** | ↑ (2) | 1.2 (0.25) | 1.6 (0.03) | 1.0 (0.84) | Scavenger recep. Cysteine-rich type 1 protein m160 precursor |
| CKB | ↑ (2) | 0.9 (0.33) | 0.9 (0.41) | 0.9 (0.38) | Creatine kinase B-type |
| DTL | ↑ (2) | 0.8 (0.03) | 0.9 (0.12) | 0.8 (0.03) | Denticleless protein homolog |
| DUOX2** | ↑ (2) | 1.3 (0.06) | 1.3 (0.05) | 1.2 (0.18) | Dual oxidase 2 precursor |
| DUSP27 | ↓D (22) | 1.0 (0.95) | 0.9 (0.81) | 1.0 (0.9) | Dual specificity phosphatase 27 (putative) |
| ERAP2 | ↑ (2) | 0.9 (0.8) | 1.4 (0.5) | 0.6 (0.27) | Endoplasmic reticulum aminopeptidase 2 |
| F2RL1 | ↑ (75) | 0.9 (0.63) | 0.9 (0.29) | 1.0 (0.82) | Proteinase-activated receptor 2 precursor |
| F2RL3*** | ↓ (130) | 1.0 (0.58) | 1.0 (0.99) | 0.9 (0.34) | PAR4 coagulation factor II (thrombin) receptor-like 3 |
| FAAH | ↓C (31) | 1.1 (0.09) | 1.2 (0.05) | 1.1 (0.31) | Fatty-acid amide hydrolase 1 |
| FCGR2A | ↑ (2) | 0.5 (0.09) | 0.7 (0.3) | 0.5 (0.05) | Low affinity Ig gamma Fc region recep, II-a isof.1 precursor |
| FGFR4* | ↓D (21) | 0.9 (0.12) | 1.0 (0.86) | 0.8 (0.01) | Fibroblast growth factor receptor 4 isof. 2 precursor |
| FN1 | ↓D (21, 22) | 0.8 (0.07) | 0.8 (0.12) | 0.8 (0.12) | Fibronectin isof. 5 preproprotein |
| FOXP3 | ↓ (13) | 0.6 (0.001) | 0.5 (0.001) | 0.7 (0.02) | Forkhead box P3 |
| GPBAR1 | ↑C (21) | 0.9 (0.2) | 1.0 (0.46) | 0.9 (0.14) | G protein-coupled bile acid receptor 1 |
| GPER1 | ↑D (98) | 1.3 (0.01) | 1.5 (0.01) | 1.2 (0.11) | G protein-coupled estrogen receptor 1 |
| GUCA2B*** | ↑D (22) | 1.2 (0.4) | 1.4 (0.25) | 1.1 (0.75) | Guanylate cyclase activator 2B (uroguanylin) |
| HELLS*** | ↑ (2) | 1.2 (0.4) | 1.6 (0.01) | 1.1 (0.75) | Helicase, lymphoid-specific |
| HLA-DRB5 | ↓D (22) | 1.2 (0.55) | 1.0 (0.73) | 1.3 (0.49) | MHC, class II, DR beta 5 precursor |
| HSPA1A*** | ↓D (22) | 0.9 (0.48) | 0.9 (0.57) | 0.9 (0.51) | Heat shock 70 kda protein 1A/1B |
| HSPA1B | ↓D (22) | 1.0 (0.92) | 1.0 (0.77) | 1.0 (0.9) | Heat shock 70 kda protein 1B |
| IFIT3** | ↓D (21, 22) | 0.9 (0.07) | 0.9 (0.19) | 0.9 (0.07) | Interferon-induced protein with tetratricopeptide repeats 3 |
| IGFBP2*** | ↑D (22) | 1.6 (0.07) | 2.1 (0.01) | 1.2 (0.61) | Insulin-like growth factor-binding protein 2 precursor |
| IL10*** | ↓ (13, 30) | 0.8 (0.28) | 0.9 (0.62) | 0.7 (0.18) | Interleukin-10 precursor |
| IL13 | ↑ (125) | 0.9 (0.14) | 0.8 (0.02) | 1.0 (0.85) | Interleukin-13 precursor |
| IL1B | ↑ (4) | 0.8 (0.31) | 0.8 (0.31) | 0.8 (0.45) | Interleukin-1 beta proprotein |
| KCNS3 | ↑ (2) | 1.0 (0.81) | 0.8 (0.26) | 1.1 (0.48) | Potassium voltage-gated channel subfamily S member 3 |
| KDELR2 | ↑ (42) | 1.1 (0.45) | 1.2 (0.1) | 1.0 (0.73) | ER lumen protein retaining receptor 2 isof. 1 |
| LCN15*** | ↑D (22) | 1.0 (0.9) | 0.8 (0.16) | 1.2 (0.23) | Lipocalin 15 |
| LYZ | ↓ (2) | 1.0 (0.96) | 1.3 (0.32) | 0.8 (0.36) | Lysozyme C precursor |
| MCM5*** | ↑ (2) | 1.0 (0.7) | 1.2 (0.1) | 0.9 (0.32) | DNA replication licensing factor MCM5 |
| MS4A4A** | ↑ (2) | 1.4 (0.07) | 1.8 (0.01) | 1.1 (0.71) | Membrane-spanning 4-domains subfam. A member 4a isof. 1 |
| MUC20* | ↑ (2) | 1.2 (0.04) | 1.3 (0.01) | 1.1 (0.49) | Mucin-20 isof. S |
| MX1*** | ↓D (22) | 1.0 (0.94) | 1.1 (0.76) | 0.9 (0.67) | Interferon-induced GTP-binding protein Mx1 |
| NCF4* | ↑ (2) | 1.3 (0.04) | 1.6 (0.001) | 1.0 (0.99) | Neutrophil cytosol factor 4 isof. 2 |
| NOS2 | ↑ (4) | 0.9 (0.07) | 1.0 (0.48) | 0.8 (0.02) | Nitric oxide synthase, inducible |
| NR1H4 | ↑D (21) | 1.1 (0.88) | 1.9 (0.11) | 0.6 (0.18) | Bile acid receptor isof. 2 |
| OCLN** | ↑C (21) | 1.2 (0.06) | 1.2 (0.06) | 1.1 (0.17) | Occludin isof. B precursor |
| P2RY1*** | ↑ (81) | 1.1 (0.51) | 1.1 (0.37) | 1.0 (0.79) | P2Y purinoceptor 1 |
| P2RY2 | ↑ (81) | 1.0 (0.83) | 1.0 (0.31) | 0.9 (0.17) | P2Y purinoceptor 2 |
| P2RY4*** | ↑D (21, 22) | 1.0 (0.89) | 0.9 (0.13) | 1.1 (0.2) | Pyrimidinergic receptor P2Y, G-protein coupled, 4 |
| PDZD3*** | ↑D (21, 22) | 1.1 (0.25) | 1.2 (0.25) | 1.1 (0.4) | Na(+)/H(+) exchange regulatory cofactor NHE-RF4 isof. 2 |
| PSME2*** | ↑ (2) | 1.0 (0.62) | 1.2 (0.01) | 0.9 (0.09) | Proteasome activator complex subunit 2 |
| RBP2 | ↑D (22) | 1.2 (0.33) | 1.4 (0.08) | 1.0 (0.89) | Retinol-binding protein 2 |
| RFC4*** | ↑ (2) | 1.1 (0.27) | 1.3 (0.04) | 1.0 (0.81) | Replication factor C subunit 4 |
| S100A10 | ↑ (20, 106) | 0.9 (0.11) | 0.9 (0.34) | 0.8 (0.07) | Protein S100-A10 |
| SLC10A2 | ↑D (21) | 1.0 (0.86) | 1.1 (0.33) | 0.9 (0.51) | Ileal sodium/bile acid cotransporter |
| SLC6A4 | ↓ (36, 45, 63, 76, 122) | 1.9 (0.01) | 1.3 (0.38) | 2.9 (0.001) | Sodium-dependent serotonin transporter |
| TAP2*** | ↑ (2) | 1.1 (0.79) | 1.9 (0.05) | 0.6 (0.12) | Antigen peptide transporter 2 isof. 2 |
| TFF1 | ↑D (22) | 1.0 (0.92) | 1.1 (0.62) | 0.9 (0.76) | Trefoil factor 1 precursor |
| TH | ↑D (129) | 0.9 (0.25) | 0.9 (0.37) | 0.9 (0.26) | Tyrosine 3-monooxygenase isof. B |
| TJP1*** | ↓ (21, 119) | 0.9 (0.21) | 0.9 (0.5) | 0.9 (0.13) | Tight junction protein ZO-1 isof. A |
| TLR2*** | ↑ (12) | 1.0 (0.86) | 1.1 (0.64) | 1.0 (0.86) | Toll-like receptor 2 precursor |
| TLR4*** | ↑ (12) | 1.0 (0.63) | 1.0 (0.92) | 1.1 (0.36) | Toll-like receptor 4 isoform A precursor |
| TNFSF15 | ↓ (21, 112) | 1.0 (0.88) | 1.0 (0.68) | 1.0 (0.88) | TNF ligand superfam. member 15 isof. VEGI-251 precursor |
| TPH1 | ↓ (63) | 1.3 (0.17) | 1.5 (0.12) | 1.2 (0.4) | Tryptophan 5-hydroxylase 1 |
| TPSAB1 | ↑ (4, 75) | 0.8 (0.03) | 0.9 (0.17) | 0.8 (0.02) | Tryptase alpha/beta-1 precursor |
| TRPV1 | ↑ (64, 81, 131) | 0.8 (0.05) | 0.7 (0.08) | 0.8 (0.1) | TRP cation channel subfamily V member 1 |
| VIP* | ↑D (21, 22) | 0.7 (0.06) | 0.6 (0.03) | 0.8 (0.25) | VIP peptides isoform 2 preproprotein |
| VSIG2 | ↑ (2) | 0.8 (0.07) | 0.8 (0.16) | 0.8 (0.08) | V-set and Ig domain-containing protein 2 precursor |
| VSIG4* | ↑ (2) | 1.4 (0.03) | 1.7 (0) | 1.1 (0.5) | V-set and Ig domain-containing protein 4 isof. 1 precursor |
| WDR72*** | ↓D (14) | 0.8 (0.02) | 0.8 (0.01) | 0.9 (0.15) | WD repeat-containing protein 72 |
↑, increased vs. healthy controls (HCs); ↓ decreased vs. HCs; D, IBS with diarrhea; C, IBS with constipation; isof., isoform; dep., dependent; sub., subunit; Ig, immunoglobulin; recep., receptor; subfam., subfamily; MHC, major histocompatibility complex; TNF, tumor necrosis factor; TRP, transient receptor potential.
*
Our results agree with reported findings at P < 0.05,
**
P < 0.1 and
***
not significant but same direction, respectively.