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. 2018 Aug 20;9:1076. doi: 10.3389/fphys.2018.01076

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Copyright © 2018 Thorrez, DiSano, Shansky and Vandenburgh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Collagen deposition induced by medium supplementation with ascorbic acid, proline, and hydroxyproline (+supplements) and in the absence (stopped at day 5) or presence of aprotinin. Images from Trichome stained sections from different BAM groups, blue areas are collagen staining. (A–D) Fibrin BAMs with 1 mg/ml fibrin, additions to the media as shown on the graph. (E) 1 mg/ml collagen (Zyderm) BAM as positive control for the collagen staining. Scale bars are 100 μm. (F) Depicts collagen quantification. ^∗∗P < 0.01 and ^∗∗∗P < 0.001.