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Collagen deposition induced by medium supplementation with ascorbic acid, proline, and hydroxyproline in function of decreasing fibrin concentrations. BAMs were engineered with (A) 1 mg/ml fibrin, (B) 0.5 mg/ml fibrin, (C) 0.25 mg/ml fibrin. Images from Trichome stained sections from different BAM groups; blue areas are collagen staining. Scale bar is 100 μm. (D) Collagen quantification depicted as the percentage of blue area compared to the total area of each image. ∗∗∗P < 0.001.
