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. 2018 Aug 8;9:1104. doi: 10.3389/fphys.2018.01104

FIGURE 3.

FIGURE 3

Sch inhibits LPS-induced leukocyte adhesion to the pulmonary venules and the expression of adhesion molecules 90 min after LPS infusion. (A) Representative images showing leukocyte adhesion to the pulmonary venular wall in Sham (a), Sch alone (b), 90 min after LPS infusion (c), and Sch pretreatment at 2.5 mg/kg/h followed by LPS infusion for 90 min (d) group. Arrows indicate adherent leukocytes. Bar = 50 μm. (B) Statistical result of the number of leukocytes adherent to the pulmonary venules (N = 6). (C) Representative images of immunohistochemical staining for MPO in Sham (a), Sch alone (b), 90 min after LPS instillation (c), and Sch pretreatment at 2.5 mg/kg/h followed by LPS infusion for 90 min (d) group. All images are representative of 3 separate experiments. Arrows indicate recruited leukocytes in pulmonary venules. Bar = 50 μm. (D) Quantification of MPO activity in rat lung tissues in different groups (N = 5). (E) The expression of CD11b and CD18 on neutrophils measured by flow cytometry (N = 6). (F,G) The expression of ICAM-1 (F) and VCAM-1 (G) in the lung tissue assessed by Western blotting (N = 5). Sham, sham group; Sch, sch alone group; LPS, LPS infusion for 90 min group; Sch + LPS, Sch pretreatment at 2.5 mg/kg/h followed by LPS infusion for 90 min group. Values are expressed as mean ± SD. ∗p < 0.05 vs. Sham group, #p < 0.05 vs. LPS group.