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. 2018 Aug 8;9:1104. doi: 10.3389/fphys.2018.01104

FIGURE 6.

FIGURE 6

Sch recovers the expression of endothelial tight junction and adherent junction protein at 8 h after the termination of LPS infusion. (A,B) Representative Western blotting images of ZO-1, VE-Cadherin, Occludin, and Claudin-5 at 90 min after LPS infusion (A), and 8 h after the termination of LPS infusion (B), respectively, among different groups. (C–F) Quantitative analysis of ZO-1 (C), VE-Cadherin (D), Occludin (E), and Claudin-5 (F) at each time point after the LPS administration in different groups. Values are expressed as mean ± SD of 5 separate experiments. (G) Representative immunofluorescence confocal images for Claudin-5 in Sham (a), Sch alone (b), 8 h after the termination of LPS infusion (c), and 8 h after the termination of LPS infusion in the presence of Sch pretreatment (2.5 mg/kg/h) (d) group. All images are representative of 3 separate experiments. Bar = 5 μm. Sham, sham group; Sch, sch alone group; LPS, LPS infusion for 90 min group; Sch + LPS, Sch pretreatment at 2.5 mg/kg/h followed by LPS infusion for 90 min group. ∗p < 0.05 vs. Sham group, #p < 0.05 vs. LPS group.