Table 2.
The number of animals for different experimental groups and various parameters at 8 h after the termination of LPS infusion.
Groups | Sham | Sch | LPS | Sch 1.25 + LPS | Sch 2.5 + LPS | Sch 5 + LPS | Total |
---|---|---|---|---|---|---|---|
Evans blue extravasation | 6 | 6 | 6 | 6 | 6 | 6 | 36 |
Wet-to-dry weight ratio | 6 | 6 | 6 | 6 | 6 | 6 | 36 |
Histology and immunohistochemistry | (3) | (3) | (3) | (3) | (12) | ||
MPO activity assay | (5) | (5) | (5) | (5) | (20) | ||
Western blotting | (5) | (5) | (5) | (5) | (20) | ||
Bronchoalveolar lavage fluid ELISA | 6 | 6 | 6 | 6 | 24 | ||
Total | 18 | 18 | 18 | 12 | 18 | 12 | 96 |
Sham, sham group; Sch, sch alone group; LPS, LPS infusion for 90 min group; Sch 1.25 + LPS, Sch 2.5 + LPS, and Sch 5 + LPS represent Sch pretreatment at 1.25, 2.5, and 5 mg/kg/h followed by LPS infusion for 90 min group, respectively. The same rat was used for assessment of wet-to-dry weight ratio, histology and immunohistochemistry, MPO activity assay and Western blotting. Number in parentheses means the same rats were used as the upper row.