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Stern-Volmer (KSV) constants for fluorescence quenching in MSG by acrylamide
The values are calculated by nonlinear least-square fitting of the data (Fig. 5B) in the Stern-Volmer equation (F0/F = 1 + KSV × [quencher]). The errors shown represent standard error of fitting.
| Conformational state | KSV |
|---|---|
| m−1 | |
| Native | 2.01 ± 0.07 |
| Misfolded state (IM) in 1 m urea | 4.39 ± 0.09 |
| Unfolded (6.5 m urea) | 9.10 ± 0.29 |
