Skip to main content
. 2018 Jul 5;293(34):13257–13269. doi: 10.1074/jbc.RA118.002428

Figure 2.

Figure 2.

SAA triggers inflammasome priming and activation in macrophages. A, J774 cells were incubated ±50 μg/ml of mouse SAA for 8 h prior to quantification of IL-1β mRNA by qRT-PCR. B, J774 cells were incubated with the indicated concentrations of mouse SAA for 24 h and IL-1β in the media was quantified by ELISA. C, J774 cells were incubated with 5 μg/ml of mouse SAA for the indicated times and IL-1β in the media was quantified by ELISA. D, IL-1β mRNA abundance was determined in untreated J774 cells (control), or cells treated for 8 h with 25 μg/ml of human SAA (hSAA) as indicated. E, IL-1β levels were determined by ELISA in conditioned media from J774 cells incubated for 24 h ±25 μg/ml of hSAA. Data are presented as mean ± S.E. ***, p < 0.001. Data that are not significantly different (p > 0.05) are indicated with the same letter.