Figure 6.

SAA-mediated IL-1β production depends on cathepsin B activity. A, IL-1β levels in conditioned media from untreated J774 cells or cells incubated for 24 h with 5 μg/ml of SAA in the presence or absence of specific inhibitors for cathepsin B (12.5 μm CA-074-Me) or cathepsin L inhibitor (15 μm), as indicated, were determined by ELISA. B, IL-1β levels in conditioned media from untreated J774 cells or cells incubated for 24 h with 5 μg/ml of SAA with and without 3 μm cytochalasin D (CytD) were determined by ELISA. C, IL-1β levels in conditioned media from J774 cells treated with 500 ng/ml of LPS for 3 h followed by a 4-h incubation with alum (200 μg/ml) in the presence or absence of 3 μm cytochalasin D were determined by ELISA. Data are presented as mean ± S.E. Data that are not significantly different (p > 0.05) are indicated with the same letter.