Figure 8.

HDL abrogates SAA-mediated inflammasome activation and ROS generation. A, IL-1β levels in conditioned media from J774 cells primed with 0.5 μg/ml of LPS for 3 h prior to 24-h incubations with 5 μg/ml of SAA ± 14 μg/ml of HDL were determined by ELISA. B, IL-1β mRNA abundance was determined for untreated J774 cells (control), or cells treated for 3 h with 0.5 μg/ml of LPS ± 70 μg/ml of HDL, as indicated. C, IL-1β levels in conditioned media from untreated J774 cells (control), cells primed with 0.5 μg/ml of LPS alone, or LPS followed by 3 mm ATP ± 70 μg of HDL for 45 min were determined by ELISA. D, J774 cells were incubated ±5 μg/ml of SAA for 24 h and activation of caspase-1 was determined by FLICA, with activated caspase-1 visualized as green fluorescence. E, intracellular ROS levels were quantified using DCFDA reagent in J774 cells that were left untreated (control) or treated with 5 μg/ml of SAA ± 14 μg/ml of HDL. Data are presented as mean ± S.E. ***, p < 0.001. Data that are not significantly different (p > 0.05) are indicated with the same letter.