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. 2018 Jun 26;293(34):13134–13150. doi: 10.1074/jbc.RA118.002073

Figure 9.

Figure 9.

Role of IDRs in N terminus in mediating heterodimerization of FLZ proteins and interaction with SnRK1 subunits. A, sequence alignment of the N terminus of FLZ1 and FLZ2 showing the position of IDRs. The conserved residues are marked by violet color. B, interaction analysis of FLZ2NIDR1 and FLZ2NIDR2 with FLZ7 and FLZ10. C, interaction analysis of FLZ2NIDR1 and FLZ2NIDR2 with SnRK1β and βγ subunits. D, interaction analysis of FLZ1NIDR1 (1–48) and FLZ1NIDR1 (49–81) with FLZ7 and FLZ15. E, interaction analysis of FLZ1NIDR1 (1–48) and FLZ1NIDR1 (49–81) with SnRK1β subunits. F, interaction analysis of FLZ1N and FLZ1N-FLZ2NIDR2 with FLZ10. The constructs prepared in AD and BD were cotransformed in Y2H Gold strain and screened on DDO (1st column in each group) and QDO plates supplemented with X-α-Gal and AbA (2nd column in each group).