Figure 5.
Inhibition of invasion of U87MG cells by N-TIMP2 variants. A, untreated cells. Cells were treated with the following: N-TIMP2WT (B); FL-TIMP2 (C); N-TIMP25M (D); N-TIMP2WT, RGD (E); N-TIMP25M, RGD (F); Ala–N-TIMP2WT, RGD (G); N-TIMP2HD (H); N-TIMP25M and Ala–N-TIMP2WT, RGD (I), and cRGD (J). K, quantification of invasive cells normalized to untreated cells. Error bars represent S.D. One-way ANOVA with Dunnett's multiple comparison with the untreated control was utilized for statistical analysis; *, p < 0.05; **, p < 0.005, n = 3. A comparison between treatment with N-TIMP2HD versus all other treatments showed the statistically significant superior inhibition ability of N-TIMP2HD when compared with N-TIMP2WT, FL-TIMP2, and Ala–N-TIMP2WT, RGD (p < 0.05), N-TIMP25M and cRGD (p < 0.005), and N-TIMP25M, RGD (p < 0.001).