Table 2.

Summary of the variants' activities in different cellular assays

ND means inhibition was not detected.

Variant	Tube formation inhibitiona,b	Invasion inhibitionb of TIME cells	Invasion inhibitionb of U87MG cells	Matured MMP-2 percentagec	Intermediate MMP-2 percentage	Total active MMP-2
	%	%	%	%	%	%
FL-TIMP2	−10d	−11d	−5d	3	0.6	3.6
N-TIMP2WT	61	8	10	1.3	4.1	5.4
N-TIMP25M	1	8	8	2.5	0.5	3
N-TIMP2WT, RGD	82	18	20	2.3	4.7	7
N-TIMP25M, RGD	45	14	−2.5	1.7	1.1	2.8
Ala- N-TIMP2WT, RGD	92	8	13	2.4	4.9	7.3
N-TIMP2HD	79	28	33	1.6	0.4	2
cRGD	ND	21	12	2.8	3.5	6.3

^a Tube formation is measured as number of segments.

^b Percent of inhibition was compared with the untreated control.

^c For untreated cells, the percentages of the different MMP-2 forms are as follows: 3.9% for MMP-2 intermediate; 3.5% for MMP-2 matured; and 7.4% for total active MMP-2 (intermediate + matured).

^d − represents activation.